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hindiii digested sgrna plasmids  (New England Biolabs)


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    Structured Review

    New England Biolabs hindiii digested sgrna plasmids
    Hindiii Digested Sgrna Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hindiii+digested+sgrna+plasmids/HindIII/pm37402729-258-18-28
    Average 99 stars, based on 10778 article reviews
    hindiii digested sgrna plasmids - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Generated:

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues.
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sasgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sa-sgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..

    Cloning:

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues.
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sasgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sa-sgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..

    Sequencing:

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues.
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sasgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..

    Article Title: High-efficiency targeted transgene integration via primed micro-homologues
    Article Snippet: All sgRNA plasmids were generated by integrating the annealed spacer oligos into BsaI digested backbones (pUC19-U6-sp-sgRNA Scaffold and pUC19-U6-sa-sgRNA Scaffold) with T4 ligase (NEB). .. PegRNA plasmids were generated by cloning the extended fragment (containing an RT-template and a PBS sequence) into corresponding HindIII digested sgRNA plasmids using HiFi DNA Assembly Master Mix (NEB). ..



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    99
    New England Biolabs hindiii digested sgrna plasmids
    Hindiii Digested Sgrna Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hindiii+digested+sgrna+plasmids/HindIII/pm37402729-258-18-28
    Average 99 stars, based on 1 article reviews
    hindiii digested sgrna plasmids - by Bioz Stars, 2026-09
    99/100 stars
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